cd40 pe vio 770 clone rea965  (Miltenyi Biotec)

Journal: Journal of Neuroinflammation

Article Title: Microglial clearance, neuroprotection and cognitive recovery via a novel synthetic sulfolipid in Alzheimer’s disease

doi: 10.1186/s12974-025-03634-w

Figure Lengend Snippet: SULF A-dependent modulation of microglial activation and arginine metabolism. ( A ) RT-PCR analysis of cytokine and microglial marker gene expression following 24 h treatment. ( B ) CCL2 secretion quantified by ELISA at 48 h (n = 12). ( C ) Flow cytometric analysis of surface marker expression (CD40, CD86, MHC II, CD200R, TREM2) on untreated microglia (CTRL) or cells treated with 10 µg/mL SULF A for 24 h, expressed as mean fluorescence intensity (MFI; n = 6). ( D ) Schematic of iNOS/ARG metabolic pathways. ( E ) Quantification of nitric oxide (NO) release in culture supernatants by Griess assay at 48 h (n = 12). ( F ) Frequency of microglial cells expressing ARG, iNOS, or both, after 48 h of SULF A treatment (n = 6); ( G–H ) Representative FACS plots showing ARG⁺, iNOS⁺, and ARG⁺iNOS⁺ populations in untreated and SULF A-treated microglia. Data represent mean ± SEM. Statistical significance determined by paired t-test (P < 0.05; **P < 0.001; ***P < 0.0001)

Article Snippet: Surface markers were assessed by staining with anti-mouse CD11b-VioBlue, CD40-PE, CD86-FITC, CD200R-APC (Miltenyi Biotec), and TREM2-APC (R&D Systems) according to standard protocols.

Techniques: Activation Assay, Reverse Transcription Polymerase Chain Reaction, Marker, Gene Expression, Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence, Griess Assay

Journal: Frontiers in Pharmacology

Article Title: The immunostimulatory activity of Epimedium flavonoids involves toll-like receptor 7/8

doi: 10.3389/fphar.2025.1514284

Figure Lengend Snippet: Effects of Epimedium flavonoids on the maturation of RAW 264.7 cells. Cell was assessed 24 h after compounds treatment in RAW 264.7 cells. The experiment was repeated three times for consistency. (A–C) . Effects of Epimedium flavonoids on the expression of CD40, CD80, and CD86, and (D, E) effects on MHC-I and MHC-II in RAW 264.7 cells, as detected by flow cytometry. RAW264.7 cells were gated on size and complexity using a two-dimensional FSC vs. SSC plot, then assessed for MHC-I, MHC-II, CD40, CD80 and CD86. Statistical analyses were performed using one-way ANOVA, followed by Tukey’s multiple comparisons test to adjust for multiple comparisons. All data are expressed as mean ± SD (n = 3). Statistical significance is indicated as follows: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the control group.

Article Snippet: Fluorophore-conjugated antibodies, including PE-CD40, PE-CD80, PE-CD86, PE-MHC-I, PE-MHC-II and PE-CD11c were acquired from Thermo Fisher Scientific (Waltham, MA, United States) and eBioscience (San Diego, CA, United States), IL-4 and IFN-γ enzyme-linked immunosorbent assay (ELISA) were obtained from Universal Biotech Co., Ltd (Shanghai, China).

Techniques: Expressing, Flow Cytometry, Control

Journal: Frontiers in Pharmacology

Article Title: The immunostimulatory activity of Epimedium flavonoids involves toll-like receptor 7/8

doi: 10.3389/fphar.2025.1514284

Figure Lengend Snippet: Effects of icaritin, icariin I and icariin II on the maturation of BMDCs. Cell was assessed 24 h after compounds treatment in BMDCs. The experiment was repeated three times for consistency. (A–C) Effects of Epimedium flavonoids on the expression of CD40, CD80, and CD86, and (D, E) effects on MHC-I and MHC-II in BMDCs, as assessed by flow cytometry. BMDCs cells were selected using a two-dimensional FSC vs. SSC plot, followed by gating on CD11c for DCs. Then assessed for MHC-I, MHC-II, CD40, CD80 and CD86. Statistical analyses were performed using one-way ANOVA, followed by Tukey’s multiple comparisons test to adjust for multiple comparisons. All data are presented as mean ± SD (n = 3). Statistical significance is indicated as follows: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, compared with the control group.

Article Snippet: Fluorophore-conjugated antibodies, including PE-CD40, PE-CD80, PE-CD86, PE-MHC-I, PE-MHC-II and PE-CD11c were acquired from Thermo Fisher Scientific (Waltham, MA, United States) and eBioscience (San Diego, CA, United States), IL-4 and IFN-γ enzyme-linked immunosorbent assay (ELISA) were obtained from Universal Biotech Co., Ltd (Shanghai, China).

Techniques: Expressing, Flow Cytometry, Control